WITHDRAWAL TIME ~ OXYTETRACYCLINE

ABSTRACT

UDE SOJUNG. 2013. Determination of Withdrawal Time  of Oxytetracycline and its Effication On Tilapia (Oreochromis niloticus) infected by Bacteria Streptococcus iniae. Supervised by MUSTAHAL and DODI HERMAWAN

The purpose of this study was to determine the withdrawal time of ocxytetracycline (OTC) until no longer content of residues discovered in fish meat below the maximum residue limit (MRL). Also to determine the effectiveness of OTC medication in tilapia that was infected by sterptococcus iniae, through study withdrawal time (WT ) and drug effication test. Withdrawal time test was done by giving  75 mg OTC / kg body weight in tilapia (Oreochromis niloticus), mixed into the feed (orally) and administerd during 10 days for 48 day observation period. The residue of OTC was analyzed by using ELISA method. Drug effication test was carried out by using tilapia fish infected by bacteria S. iniae. The bacteria were injected intra-peritoneally (IP) about 0.1 ml at 10⁷ cells / ml,. Tilapia were fed with pellet at 75 mg / kg body weight for 10 days and observed  during 14 days. The results showed that the concentration of WT = 1 MRL (100 ppb) values ​​obtained on the day 5. the intersection of the linear regression line between the upper tolerance limit with 95% confidence limit of If the withdrawal time is determined by the concentration limit WT = 0.5 MRL (50 ppb), the time the drug was stopped 14 days after the last drug delivery applications. The observation parameter test fish survival rate of infected bacteria S. iniae showed a value of 60.00 ± 8.16%. Based on the results of analysis of variance the three treatments were (negative control, infected fish without medication and infected fish with medication) significantly different (P <0.05) in the decreasing the survival  rate of tilapia.

Key words : Ocxytetracycline, Streptococcus iniae, Tilapia, Withdrawal time