ABSTRACT
UDE SOJUNG. 2013.
Determination of Withdrawal Time of Oxytetracycline and its Effication On
Tilapia (Oreochromis niloticus)
infected by Bacteria Streptococcus iniae. Supervised by MUSTAHAL and DODI
HERMAWAN
The purpose of this study was to determine
the withdrawal time of ocxytetracycline (OTC) until no longer content of
residues discovered in fish meat below the maximum residue limit (MRL). Also to
determine the effectiveness of OTC medication in tilapia that was infected by sterptococcus iniae, through study
withdrawal time (WT ) and drug effication test. Withdrawal time test was done
by giving 75 mg OTC / kg body weight in
tilapia (Oreochromis niloticus),
mixed into the feed (orally) and administerd during 10 days for 48 day
observation period. The residue of OTC was analyzed by using ELISA method. Drug
effication test was carried out by using tilapia fish infected by bacteria S. iniae. The bacteria were injected
intra-peritoneally (IP) about 0.1 ml at 107 cells / ml,. Tilapia
were fed with pellet at 75 mg / kg body weight for 10 days and observed during 14 days. The results showed that the
concentration of WT = 1 MRL (100 ppb) values obtained on the day 5. the intersection
of the linear regression line between the upper tolerance limit with 95%
confidence limit of If the withdrawal time is determined by the concentration
limit WT = 0.5 MRL (50 ppb), the time the drug was stopped 14 days after the
last drug delivery applications. The observation parameter test fish survival
rate of infected bacteria S. iniae
showed a value of 60.00 ± 8.16%. Based on the results of analysis of variance
the three treatments were (negative control, infected fish without medication
and infected fish with medication) significantly different (P <0.05) in the
decreasing the survival rate of tilapia.
Key words :
Ocxytetracycline, Streptococcus iniae,
Tilapia, Withdrawal time
